FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52778284
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24450.011.869626
ATAGGGG84500.010.81072138
AGCTTAG97050.09.29486745
GGCATAG97750.08.99954735
TGTCGTT35400.08.45216228
CATAGGG106300.08.44522837
GCATAGG107850.08.38619436
ATATAGC44600.08.3750346
AAGCCGG194150.08.11063745
GGGAGGT170050.08.0331443
GTCACTC168150.07.88208878
TAATCGG27750.07.865764
GAGCTTA118300.07.64498844
GGTCACT173200.07.54843437
GCGTGAA209150.07.476691240
TAGCGTT200600.07.390333724
CGTAGGG49400.07.241723537
AGGGATC134200.07.21163942
GGCGTAG50750.07.137571335
GCGTTTT212200.07.13501526
Produced by FastQC (version 0.11.5)