FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46221832
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA560650.12129549516773806No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG541690.11719353746082588No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG483130.10452419973314775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG157900.012.69626345
ATAGGGG161450.012.6540138
GGCATAG164150.012.15802635
GGGAGGT261250.011.9716473
GGAGGTC259550.011.8679934
GTTGTCG20100.011.30591326
TGGCATA183500.011.09667534
GTCACTC271100.010.9470838
GAGCTTA184800.010.86033544
ACTCTCC275300.010.65742211
GGTCACT283950.010.4041377
AGGGATC152200.010.3041822
GAGGTCA310050.010.2687985
CATAGGG201850.010.07673937
TCACTCT304500.09.9975529
GCATAGG208850.09.74977236
CACTCTC308400.09.46252210
AAGCCGG251200.09.36898145
TAGGGGA320350.09.14463939
GCTCTGT328100.09.09321922
Produced by FastQC (version 0.11.5)