FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50193783
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1321870.26335333202520317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT401400.024.2420432
TTCACGC396500.023.6781777
TCACGCT403600.023.2839248
GCCGGCT404200.022.4404341
ACGCTCA424200.022.41298710
AACGCTA415300.022.15296723
ACGCTAC418400.021.98933624
CGCTACC392650.021.98921825
GAAAACG412000.021.87065720
AAAACGC431150.021.41568821
CGGCTTC480600.021.047233
AAACGCT443450.020.81770722
CACGCTC455350.020.6574889
GGCTTCA540600.018.3364374
CGCTCAG528700.018.18713211
CTACCTC485450.017.91736227
TACCTCT490100.017.78496728
CGTTACG16200.017.4902921
GCTACCT507800.017.2480126
CGTGGTT537050.017.15295441
Produced by FastQC (version 0.11.5)