FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50193783
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA547090.10899557022828904No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG527950.1051823489773624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG156250.013.26901745
GGGAGGT232950.012.9314993
ATAGGGG165050.012.55613538
GGCATAG163600.012.43360435
GGAGGTC252400.011.73894
GAGCTTA183500.011.34492344
TGGCATA183650.011.28356734
CATAGGG195500.010.51989437
AGGGATC186450.010.364822
CGTGGCG127400.010.1738691
GCATAGG208250.09.90823436
GTTGTCG16600.09.75790826
CACACTC170550.09.73502324
TGGGAGG232950.09.6479222
CACCGAA321400.09.62710345
GTCACTC306450.09.4115128
GTGGCGA142700.09.1282092
GCACCGA392900.09.09056244
CAGGGAT231150.09.0828231
CGACCCG145550.09.0267546
Produced by FastQC (version 0.11.5)