FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48598846
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG15550.017.07203921
GTCCGTT41200.016.0122851
GAACGAT113550.015.25814445
AGACCGC112550.015.133824
GTTCCTA75450.014.93849815
CAGACCG119650.014.4050313
CCTACTC79450.014.32798218
CCCGTAT32200.013.8449761
ACGCTAC82050.013.43604324
AACGCTA81600.013.34459923
CCCGGAT130050.013.3310171
AGACGCT180250.013.28331741
CTCCGAT46450.013.1361011
CCCCGCA175750.012.81630435
CGCTACC86550.012.6339525
CCGCAGA195900.012.45234337
TTCGACG14400.012.3421626
CTCCGTT67600.012.2903021
CTCGGTT82950.012.2417371
GACCGCG139900.012.2234565
Produced by FastQC (version 0.11.5)