FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48598846
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG110450.011.45049345
ATAGGGG113850.010.98938738
GGCATAG117500.010.62898335
GTTGTCG17750.010.5207726
GGGAGGT212650.09.4054213
CATAGGG135850.09.37546837
GAGCTTA140000.09.32284844
GTCACTC200000.09.2683228
TAGGCTA57150.09.25082710
GGTCACT196500.09.1929917
TGGCATA142350.08.89992234
GCATAGG145800.08.75107236
ACTCTCC221500.08.61322511
TTAACGG50250.08.3275793
GAGGTCA252200.08.18014055
ATAGCTC52550.08.17874239
GCGTTCG19700.08.10884515
GGAGGTC248800.08.0839834
TATCCTA71550.08.0180195
CACTCGA73950.08.0011815
Produced by FastQC (version 0.11.5)