FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44471207
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG86550.011.80972645
ATAGGGG90950.011.50264138
GGCATAG90100.011.46212235
CATAGGG98650.010.40027737
CGTAGGG50850.010.35385437
GGCGTAG52300.010.06679735
GCGTAGG52100.010.0190736
GCATAGG105000.09.68559536
GAGCTTA109250.09.33595344
GGGAGGT190800.09.3239153
TGGCATA113550.09.23383834
GTTGTCG16000.09.14237526
TGGCGTA60450.08.78413934
GCGTTCG15200.08.58454115
GGTCACT189400.08.44862657
ACACTCG76950.08.3916624
TCAGACG66250.08.3144642
CACTCGA72250.08.2837745
GTCACTC195450.08.2564848
ACTCTCC200050.08.23517711
Produced by FastQC (version 0.11.5)