FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50476592
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT721530.14294348556653746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT146100.023.42310745
AGACCGC159900.021.4342654
CAGACCG169450.020.359023
AGAACGA182150.018.8243744
TTCGGCG77550.017.37839544
ATACGGA197200.017.31923333
TACGGAG197500.017.21318234
GCGTTCT200950.017.119219
CGTTACG17500.017.09926421
CCCGTAT25000.016.751411
AATACGG207600.016.48411632
GTCCGTT29650.016.4024011
ACGTTCG20250.015.9993276
CTCAGAC246500.015.7287651
CTCCGAT41000.015.5410721
CGTACGC87650.015.4304585
GACCGCG224400.015.3034035
CCGTACG88050.015.2837164
CGGATAC67250.015.2256353
ACGGAGA224300.015.20665635