FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50476592
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC92650.016.5043665
TCACCCG103300.015.3918243
AGCTTAG134750.013.70886145
ATAGCTC109550.013.22560739
GGCATAG143550.012.7236535
ACACGGA121300.012.57799716
CACCCGG126950.012.5775924
ACGGACA123250.012.43344818
CGGACAG122550.012.30259619
GAGCTTA153550.012.04507344
TAGCTCT120550.012.00018740
CACGGAC126900.011.98724717
ATAGGGG163500.011.58589538
GACACGG130900.011.517858515
CGGACAC151850.011.394958513
CATAGGG165150.011.3602837
CCGGACA153450.011.28921512
TGATAGC134200.011.09703837
CGGCCCG142200.011.0729078
GGGAGGT273650.011.0526463
Produced by FastQC (version 0.11.5)