FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49563173
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG794410.1602823128373964No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT586400.11831365195283199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC57800.023.00772732
CGTCCCG68350.020.01862735
TTCGACG24350.019.9570986
TCGTATG68500.019.9050745
AGACCGC158650.017.8553944
GACCGCG159750.017.5775185
CAGACCG163750.017.4779153
GCCGGCT252600.017.2300661
ACCGCGT164900.017.1633746
CCGGCTT295900.016.9948832
CGTCTGA80400.016.87316116
TCACGCT285900.016.8399568
CCCGTAT43900.015.9508681
TTCACGC306600.015.798457
ACACGTC87250.015.65158813
CCGCGTT183150.015.4776947
CGGCTTC341950.015.3114953
TCGACGA33800.015.3092447
ACGTCTG89100.015.25086315
GTCCGTT47150.015.233421
Produced by FastQC (version 0.11.5)