FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49563173
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA718520.14497054092965356No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG640320.12919269716650306No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG601230.12130579291200748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT56900.023.13027211
TCGTGTA63200.020.78905114
GTCGTGT69800.019.01663813
GTCGCCG72000.018.81803344
GGTCGCC73300.018.26915443
CGTCGTG73200.018.01039712
CGCCGTA18400.017.73608645
TGTAGGG77050.017.31511517
GTAGGGA77800.017.14828118
TCTCGGT80500.016.18498436
AGCGTCG83550.016.07552110
TCGCCGT83500.015.33675345
AGTGTAG93300.014.39672128
GTTGTCG28850.014.3493426
ATCTCGG99550.014.15013135
AGAGCGT97050.014.1175798
TGGTCGC104450.014.1121242
GTGTAGG97650.014.03089216
GTAGATC96150.013.85393431
TAGATCT102550.013.12133532
Produced by FastQC (version 0.11.5)