FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46223582
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT514100.11122028578399658No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG494600.10700165988866894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33600.022.4990546
CGGTATA54750.020.2183320
TCGACGA38500.019.4602227
GTTCGAC42150.017.6690045
CGTTACG23100.017.33693521
CGACGAG42600.017.2175878
ACCGCGT147550.016.102346
CCGGCTT196150.016.0820482
GCCGGCT172600.016.073371
AGACCGC148450.015.89926054
GACCGCG149150.015.8698975
GTCCGTT33200.015.3287711
CAGACCG155350.015.2944673
GACGGTA73600.014.85677818
ATGACGG68500.014.78054516
CCGCGTT162150.014.6663597
TCACGCT197600.014.6426038
TTCACGC196900.014.62617
CGCGTTC164000.014.5009158
CCCGTAT31100.014.2640491
Produced by FastQC (version 0.11.5)