FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48334116
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1268270.26239644064246465No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1049260.21708476058608378No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC1048310.21688821204467668No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT653910.13528953338052152No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG499220.10328522404340654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC248450.021.8143024
CAGACCG254850.021.328283
ACCGCGT253050.021.239926
GACCGCG260500.020.6843015
GCCGGCT343700.020.1307181
CCGCGTT269950.019.943557
CGCGTTC273200.019.7310058
TCAGACC288750.019.2917732
CCGGCTT384100.019.1768782
TCACGCT379850.018.621458
TTCACGC380600.018.5788447
CGGCTTC416400.018.0675433
CGTTACG18950.017.57365421
GCGTTCT311150.017.3897139
CTCAGAC350300.017.3483661
GTTCCTA76000.017.34728815
GAACGAT313500.017.0886145
CACGCTC419550.016.9399859
TTCGACG15600.016.0081626
ACGCTCA456900.015.97867210
Produced by FastQC (version 0.11.5)