FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54999187
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1728150.3142137355594002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT330350.025.6415145
GACCGCG375850.024.1959275
AGACCGC380050.024.0528534
ACCGCGT381150.023.8948926
CAGACCG384900.023.860833
CCGCGTT390650.023.278877
CGCGTTC394750.023.0655848
TCAGACC431750.021.600342
AGAACGA402500.021.03396844
CTCAGAC483450.020.384311
GCGTTCT458100.020.0477719
AATACGG441150.019.55402632
TACGGAG441350.019.4788434
ATACGGA444800.019.45930933
AAGAACG435200.019.44817243
CAATACG462000.018.76408631
TTCGACG33800.018.371856
ACGGAGA470100.018.31153135
CGGAGAG479000.017.9107736
CGTTCTC521100.017.8053710
Produced by FastQC (version 0.11.5)