FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54999187
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG181500.012.74230838
GGCATAG187700.012.32064735
GGGAGGT280000.012.0890953
AGCTTAG192750.011.92491445
GTTGTCG30400.011.84129826
GGTCACT274500.011.6252387
GTCACTC278350.011.5695598
GGAGGTC294350.011.35424
TGGCATA213950.010.77761334
GAGCTTA217900.010.560286544
ACTCTCC310100.010.52274511
CATAGGG224700.010.31248637
GAGGTCA334000.010.2014645
GCATAGG231950.010.05780836
CACTCTC320150.09.93910510
TCACTCT335850.09.8765649
TAATCGG50700.09.2775324
AGGTCAC376650.09.141416
TAGGGGA350550.09.1259539
GCTCTGT375400.08.75002222
Produced by FastQC (version 0.11.5)