FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49556800
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG608410.12277023536628677No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA533070.10756747812610984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG176200.017.68159545
ATAGGGG196350.016.0441138
GGCATAG194950.016.00895335
GAGCTTA208250.015.11165344
GGGAGGT312700.015.0791213
CATAGGG224050.014.05052537
TGGCATA223650.014.0148234
ACTCTCC333600.013.36896611
GCATAGG237300.013.30392436
GGTCACT338350.013.264997
GTCACTC342100.013.2120778
GGAGGTC351900.013.0951184
CACTCTC376850.012.13358110
GTTCTAT239600.012.0268691
CACCGAA247900.011.89607545
TGGGAGG285300.011.8120912
AGCATAG108500.011.75522345
GCACCGA251750.011.69632544
TGTCCAA390700.011.47669426
GCTCTGT392250.011.44266822
Produced by FastQC (version 0.11.5)