FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53316507
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1393770.26141434959345705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT244200.026.97518545
AGAACGA305050.021.63574844
AGACCGC329100.020.0433084
CAGACCG340500.019.4251523
TTCGACG33350.019.3591386
AAGAACG353250.018.7154543
GACCGCG357150.018.4427665
ACCGCGT365150.018.1308826
CCGCGTT369500.017.9173497
CGCGTTC371550.017.8663438
AATACGG375150.017.52428832
ATACGGA375100.017.51883533
TACGGAG375450.017.4476134
TCAGACC395900.016.9455592
CCGTACG91100.016.842224
CGTACGC91400.016.8350965
CAATACG393800.016.8272331
CTCAGAC434000.016.5056781
GCGTTCT406350.016.3639379
ACGGAGA407200.016.24714535
Produced by FastQC (version 0.11.5)