FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53316507
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG543560.10194966448195866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG171450.015.89818845
ATAGGGG182850.014.91645438
GGCATAG186400.014.51241935
GAGCTTA206600.013.23315144
GGTCACT297850.012.8953697
TGGCATA212150.012.82388234
CATAGGG216950.012.60369437
GTCACTC308700.012.5577128
GGGAGGT323550.012.539673
GTTGTCG26450.012.418826
ACTCTCC321500.012.14337411
GCATAGG231400.011.7582936
ATAGCTC71650.011.30736539
GGAGGTC358600.011.2827384
TCACTCT364600.010.8598629
CACTCTC360550.010.74910
AGGTCAC374450.010.7141426
GAGGTCA380800.010.6833025
ACCCGGC83550.010.66485
TCAGACG93550.010.2712292
Produced by FastQC (version 0.11.5)