FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48595026
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT734560.15115950344382983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT191050.019.42385945
AGACCGC201300.018.6674734
CAGACCG206700.018.245353
CGTTACG15200.017.74498621
TTCGACG18650.016.6327386
AACGCTA181300.015.660356523
AGAACGA238600.015.62909344
GACCGCG241000.015.6110485
TCACGCT194450.015.3158948
ACGCTAC175150.015.31279824
TTCACGC200850.015.0524987
ACCGCGT252900.014.9944226
CCGTACG85500.014.9220934
CGCTACC179950.014.82938425
GCCGGCT194550.014.8289321
CGTACGC86850.014.8196865
CCGGCTT219750.014.7247732
GTCCGTT37100.014.61827851
GCGTTCT259450.014.53699
ATACGGA259100.014.39526333
Produced by FastQC (version 0.11.5)