FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48595026
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163950.013.92242445
GGGAGGT254750.012.9920113
TAGCGTA6000.012.76242437
GGCATAG179600.012.48979735
ATAGGGG183650.012.34934938
GAGCTTA190250.011.89906544
GGTCACT269750.011.5024927
GTCACTC272900.011.4367998
ACTCTCC283050.011.19351211
CATAGGG203900.011.13393937
GGAGGTC306500.010.8350194
TGGCATA211250.010.71449634
GCATAGG213300.010.67487136
GTTGTCG23000.010.46281726
TCACTCT314700.010.2606829
GAGGTCA323800.010.2490555
CGTGGCG165350.010.229591
CACTCTC311300.010.18494110
TCAGACG94600.010.0063052
GTGGCGA167750.09.89182952
Produced by FastQC (version 0.11.5)