FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47708917
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1485890.31144911547667287No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT696510.14599157637554422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT310450.025.2204911
CCGGCTT359650.023.9824712
TCACGCT347800.023.9587048
TTCACGC352900.023.6698347
AACGCTA360800.022.79347823
ACGCTAC363250.022.58504324
CGCTACC371600.022.04793425
AAAACGC377850.021.80472421
GAAAACG383950.021.47780620
ACGCTCA391300.021.42174710
AAACGCT387850.021.27921922
CGGCTTC423950.020.7589843
CACGCTC415750.020.0591359
AGACCGC204950.019.2202974
GTTTTCG433750.018.78068245
CAGACCG216900.018.3584333
CGTGGTT444300.018.34994141
GAACGAT218450.018.3002845
CTACCTC459150.018.0056427
GACCGCG223750.017.6154175
Produced by FastQC (version 0.11.5)