FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47708917
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG683810.14332960020869895No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA502010.10522351618251992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT328750.014.2768143
AGCTTAG245850.013.44419645
GGTCACT348850.013.2736097
GGAGGTC361150.013.1704364
GTCACTC371300.012.6710178
ACTCTCC370900.012.46630711
ATAGGGG270550.012.29162338
GTTGTCG25800.012.20929826
GGCATAG271150.012.09016735
GAGCTTA282950.011.7211644
TCAGACG99500.011.5100572
TGGGAGG310400.011.3877332
TGGCATA293500.011.32282334
TCACTCT424300.011.2685449
CACTCTC412300.011.21453610
GTTCTAT135000.010.8833411
CGTGGCG161450.010.28492451
GCTCTGT450750.010.25290822
CATAGGG325050.010.20996437
TCTCCCC462100.010.10333913
Produced by FastQC (version 0.11.5)