FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45778154
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA632210.13810299122153333No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG525750.11484735710400205No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG519770.11354105716014674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG143050.015.31985845
GGCATAG149650.014.44862335
ATAGGGG156400.013.97047438
GAGCTTA168250.012.95844244
CATAGGG172500.012.75807437
GTTGTCG21150.012.5523626
TGGCATA174750.012.54081834
GGTCACT248200.012.4803867
GGGAGGT278100.012.2954023
GTCACTC252150.012.28463658
ACTCTCC255900.012.2282711
GCATAGG182500.011.96037236
GGAGGTC297350.010.932014
ATAGCTC75700.010.90911239
ACCCGGC80050.010.8475165
CACTCTC294400.010.65201510
AGGTCAC309400.010.5207796
GAGGTCA313700.010.505765
TCACTCT306900.010.4600079
TAGGGGA297450.010.30344339
Produced by FastQC (version 0.11.5)