FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48015425
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1724380.3591304252747945No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1144630.23838797636384557No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG529520.11028122733475754No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG515840.10743214289991186No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC509790.10617213114327323No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA507380.10567020910467835No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG501690.10448517325421987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT335900.024.05447845
TCACGCT284150.020.7426478
AACGCTA278350.020.72465323
TTCACGC284950.020.6449387
ACGCTAC280950.020.58092924
CCGGCTT306100.020.1697272
AGAACGA405000.020.04659344
CGCTACC290850.019.81862825
AAAACGC293600.019.73754721
ACGCTCA307000.019.68232210
AGACCGC412300.019.6020854
CAGACCG417700.019.4510153
GCCGGCT309850.019.287321
ACCGCGT421200.019.217126
GAAAACG303250.019.16665520
AAACGCT307600.018.80479422
GACCGCG431500.018.7231165
CGCGTTC435500.018.6323878
CCGCGTT437800.018.508817
CGGCTTC349800.018.0616133
Produced by FastQC (version 0.11.5)