FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45095504
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA604070.1339534868043608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT246300.013.6653921
GTTGTCG22450.013.22833526
GCACCGA265500.013.06781844
CACCGAA272750.012.74509645
TCTATAG285550.012.4313623
ATAGGGG97400.012.15079638
TTCTATA319450.011.5990432
CTATAGT306850.011.5703444
ATAGTGT313100.011.4465736
TATAGTG315450.011.2691715
GGCATAG102850.011.26587635
TTACTAG314500.011.13942414
TAGTGTG319850.011.1063497
AGCTTAG107700.011.05145345
TACTAGA321400.010.87227315
AGCACCG325850.010.66825843
CGTGTAG329400.010.45018936
GTGTTAC343150.010.33318511
GGGAGGT165850.010.1928033
GTTACTA345650.010.13539313
Produced by FastQC (version 0.11.5)