FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49531305
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT961310.19408129868575036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC216850.021.997094
CAGACCG230450.020.8649353
ACCGCGT232900.020.5001376
GACCGCG233750.020.3681475
GAACGAT241400.019.55431245
CGCGTTC245650.019.4816978
CCGCGTT245500.019.4570187
TCAGACC276150.017.8031372
GCGTTCT270800.017.6889929
CTCAGAC321900.016.8322871
AGAACGA284850.016.72164344
TACGGAG299300.015.78653834
AATACGG301300.015.77882532
ATACGGA301350.015.73887633
GTCCGTT34500.014.9356461
CGTTCTC333200.014.81520710
CCGTACG76350.014.7055184
CAATACG325000.014.70433731
ACGGAGA323200.014.57042435
CGTACGC77250.014.5341465
Produced by FastQC (version 0.11.5)