FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49531305
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27800.014.80666126
TAGCGTA7300.013.86773137
AGCTTAG155850.012.50481645
ACCCGGC77650.011.624755
GGCATAG165850.011.52919635
TGTCGTT36350.011.26276528
GGGAGGT271350.011.2578583
ATAGGGG176050.011.1185738
GAGCTTA184650.010.48130544
TCAGACG88750.010.3745442
TGGCATA189600.010.21541434
GGAGGTC279050.010.2128074
TCACCCG96750.010.07494453
GCTAAGT44200.09.97939914
ATAGCTC81600.09.92613839
ACGGACA89850.09.91526318
CATAGGG200450.09.77524937
ATAGCGT11300.09.75491136
GTCGTTC41950.09.49110329
GGTCACT296650.09.4587837
Produced by FastQC (version 0.11.5)