FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67644533
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700860.39927247335715954No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA1593270.23553566405728607No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA1256090.18568980289951886No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG1079290.15955317482936868No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT946200.13987826628945757No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG877150.1296704938446393No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG687650.10165640436899756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG13950.028.38608740
CTAGCGA15100.026.5224741
TTCGACG47300.025.97436
CGAACCG18400.024.1287543
CGAGCGT25000.023.48621229
CGGTATA74100.021.52439320
CTCGCGT25800.021.36291536
AGACCGC264350.021.022744
ACGAGAC40300.020.93554739
CCGCTAA19800.020.68091444
CAGACCG271400.020.5352863
TCGACGA61150.020.5277297
GTTCGAC57050.020.3914995
CGTTTAA58800.020.16229623
CGACGAG60050.019.5176538
TACGAGT37750.019.18853417
CGCTAAG21950.018.96297845
CCGTACG90700.018.711634
ACCGCGT284850.018.6821696
CGTTATA21950.018.6631284
Produced by FastQC (version 0.11.5)