FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67644533
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2476860.36615819344927697No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC1496050.22116347525083807No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA928800.1373059963323274No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA697100.10305341305261136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA32850.020.51969541
CGGTTCG29000.019.75878122
TCGATAG14650.019.3261133
CACGTAA20100.018.998778
ACGCTCG30800.018.23900421
GTACGTT42750.017.97700127
GTCGTTA17400.017.74472626
TCGTGTA48300.017.49781228
TAGACGC19500.017.42688834
CGTAAGT21800.017.31110610
GTCTCGT49900.017.307325
TAGGTTC50200.017.1876895
ACGTAAG22250.017.0617989
CGGTAGT19750.017.06140512
TACGCGT6900.016.9286529
CGTATCG17650.016.60566744
TTCGCGA14700.016.355920
GTAGGTT53650.016.1238754
CCGTATA16200.016.00335937
TACGTTG47850.015.96678128
Produced by FastQC (version 0.11.5)