FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52941355
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT979350.18498770951366847No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG545500.10303854142002977No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA540190.10203554480235726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG58450.027.0139186
TCGACGA67350.023.5777347
CGACGAG66950.023.1474768
CGGTATA93800.022.23001320
GTTCGAC72900.021.5667485
GAACGAT258400.019.06360445
AGTTCGA88350.017.973524
GTATAGA132450.017.07820122
GACGAGG125450.016.7280759
CGTTACG25450.016.46577621
CGAGGAT116000.016.1324511
ACGAGGA129800.016.04616510
ATGACGG121000.016.00489416
ATAGAGT139550.015.98354524
CCGGCTT213900.015.3734582
GCCGGCT197050.014.9738141
CAGTTCG115400.014.7350293
AGACCGC328900.014.6826064
GACCGCG330700.014.5006685
TTCACGC214950.014.4403147
Produced by FastQC (version 0.11.5)