FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51760312
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1299910.25114029451754466No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG684330.13221133597494544No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT533040.1029823776950958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG40000.025.7627966
GTTCGAC44050.023.291985
AGACCGC150650.022.7168244
GACCGCG152400.022.3378565
TCGACGA47600.022.2635277
GCCGGCT331550.021.5019471
ACCGCGT159500.021.4845756
CGGTATA64800.020.90243520
CAGACCG165700.020.8574333
CGACGAG49650.020.4383628
CCGCGTT172550.019.8593467
CCGGCTT416150.019.8106422
TTCACGC440500.018.9754857
CGCGTTC182000.018.828578
TCACGCT456000.018.3308588
GACGGTA76100.017.97606818
AGTTCGA61400.017.8462964
CGTTACG27500.017.3451721
ATGACGG75650.017.28012316
CGGCTTC524700.016.659893
Produced by FastQC (version 0.11.5)