FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51760312
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG806860.1558839135281874No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA748640.1446359133229336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG38600.014.80610926
GGGAGGT224100.014.31397153
GGAGGTC236650.013.6881114
ATATAGC55100.012.5742856
AGGGATC209750.012.2045792
AACTCTA107550.011.90645545
ATAGGGG218250.011.83763338
GCTAAGT52300.011.31306414
CAGGGAT260700.011.02739051
GGTCACT282850.010.9114417
TGTCGTT53150.010.88084528
GACGCAA58050.010.85552244
GGCATAG228350.010.82059935
AGCTTAG235850.010.71579745
GTCGTTC53500.010.68316929
GTCACTC286750.010.6610358
TCGTTCC56700.010.39843130
TGGGAGG260700.010.2335462
GAGCTTA248300.010.15164144
AATCGGT70150.010.1332375
Produced by FastQC (version 0.11.5)