FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43692732
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1193310.27311407306826224No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1044480.23905119963658944No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG541180.12386041687665582No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA450950.10320938502998622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30400.027.9766946
GTTCGAC36950.022.5909335
TCGACGA38350.022.2944327
AGACCGC282700.021.791264
GACCGCG282550.021.6833825
ACCGCGT286000.021.4534576
CGGTATA51500.021.1888920
CAGACCG292800.021.1010533
CGACGAG40850.020.4342658
CCGCGTT301450.020.3613827
CGCGTTC306500.019.9964928
GTTCCTA95250.019.36968415
TCAGACC334950.018.902482
GCGTTCT342050.017.9774139
CTCAGAC394100.017.5852721
GCCGGCT314950.017.5364971
CCGGCTT380050.017.4348932
ACGCGTC13700.017.24417740
AGTTCGA50600.016.985864
CCTACTC108700.016.80738618
Produced by FastQC (version 0.11.5)