FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43692732
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG839720.19218757023479327No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA613850.14049247366816064No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG532850.12195392130663746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG39050.017.34307726
GGGAGGT331900.016.5214753
GGAGGTC339000.016.049344
ATATAGC50900.014.7205656
AGCTTAG297050.014.05057345
TGGGAGG319800.013.91708852
GGTCACT391750.013.7152897
GAGGTCA404150.013.7126485
GTCACTC392600.013.6626718
GGCATAG311950.013.48043735
ATAGGGG318550.013.42009738
TGTCGTT51700.013.40419828
GTCGTTC52100.013.08535729
GCTAAGT53350.012.82090914
TGGCATA331700.012.75918734
CTGGGAG375750.012.6771741
GAGCTTA329650.012.66789844
ACTCTCC423550.012.62711511
GTTCTAT161700.012.5793721
AGGGATC185150.012.2378832
Produced by FastQC (version 0.11.5)