FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52071440
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1897950.36448963193643197No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG646660.12418707836771943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC384000.024.2385984
CAGACCG391650.023.8800413
GACCGCG396900.023.3771045
ACCGCGT403500.023.0002386
CCGCGTT420300.022.0916967
GAACGAT412800.021.99154945
CGCGTTC423650.021.9488748
TCAGACC443900.021.3734052
GCGTTCT455800.020.4747439
CTCAGAC491000.020.3307881
AGAACGA477200.019.25000844
CGTTCTC529000.017.91375710
AATACGG515000.017.82888632
TACGGAG512500.017.82387234
ATACGGA515250.017.79419133
AAGAACG527800.017.35336343
CAATACG536900.017.22736731
CACTCCC547650.017.15917824
CCTCTCA549950.017.08906219
ACTCCCC548200.017.07625225
Produced by FastQC (version 0.11.5)