FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52071440
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA611320.11740024858156409No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG551200.10585457210324893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG159800.013.182363545
GGCATAG166250.012.72718235
ATAGGGG168250.012.65221138
GGGAGGT266200.011.9664193
GTTGTCG24650.011.31267726
GTCACTC270200.011.1525578
GGTCACT268900.011.09734257
TGGCATA193400.011.05707834
GAGCTTA190800.010.99403544
AGGGATC188350.010.961652
GGAGGTC289500.010.6764414
ACTCTCC287850.010.58665711
CATAGGG204050.010.4576837
GAGGTCA312500.010.1549735
CACTCTC301750.09.99463210
TCACTCT313250.09.9579749
GCATAGG213800.09.94927636
AGGTCAC318350.09.8331526
CAGGGAT250100.09.3025361
TTACGCG16750.09.1381935
Produced by FastQC (version 0.11.5)