FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50085148
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT752980.15033997703271237No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG501460.10012149709530659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT197600.018.62820245
AGACCGC206200.017.9284974
CAGACCG216600.017.1819343
TCACGCT201500.016.32548
CCGTACG83700.016.1294964
AACGCTA192550.016.12538723
CGTACGC84950.016.0245595
ACGCTAC195100.015.92615824
GTCCGTT29750.015.8899791
GCCGGCT179350.015.62641
CGTTACG16750.015.58180521
TTCACGC213050.015.4826327
GTTCCTA69350.015.44312115
CGCTACC201350.015.40945425
CCCGTAT28650.015.4000611
ACCGCGT243100.015.3089236
AGAACGA241550.015.28537744
GACCGCG242650.015.2816775
TTCGGCG87900.015.23009844
ACGCTCA232150.014.81881110
Produced by FastQC (version 0.11.5)