FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47788999
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC96400.013.2793325
ATAGCTC97900.012.41148839
GCGTCGT29500.011.82108911
CACCCGG115050.011.38091854
TAGCTCT110000.011.10832440
CGGACAG112900.011.06062219
TCACCCG120300.010.9964553
TCGTGTA33650.010.8982614
ACACGGA113400.010.83293216
CGGCCCG126550.010.68429858
CCGGACA129850.010.39578112
AGCTTAG99500.010.155642545
CACGGAC128150.010.11303817
TGATAGC122700.010.06837137
TTGATAG126550.09.8509436
GGCATAG100400.09.74951235
TGTAGGG42750.09.05253217
GTCGTGT39950.08.89799613
GGTCACT169200.08.855377
GTTAGTT65500.08.8277321
Produced by FastQC (version 0.11.5)