FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46955183
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG505950.1077516831315512No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT481580.10256162775470387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC121350.020.3028034
CAGACCG133050.018.8218353
GACCGCG131250.018.6513945
GCCGGCT155300.018.5450331
CCGGCTT186100.017.7970942
ACCGCGT142300.017.3131146
TTCACGC190950.016.6136637
TCACGCT190700.016.399488
CCGCGTT153450.016.084437
CGCGTTC156100.015.8402038
CCCGTAT36550.015.3901111
CGTTACG16800.015.26966521
CTCCGAT42050.014.7683561
GCACTAC64600.014.52478525
ACGCTCA220400.014.43456710
TCAGACC180950.014.324572
CTCAGAC211350.014.1166091
CCCGGAT106450.014.05610751
CGGCTTC247300.014.04768853
TTCGACG14750.014.0333646
Produced by FastQC (version 0.11.5)