FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49422009
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1081180.21876488266593935No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG636790.12884745336839706No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG535170.10828576393970549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC224300.025.5281624
GACCGCG225450.025.2083365
ACCGCGT236600.024.0298796
CAGACCG245800.023.5698283
CCGCGTT247550.023.0210657
CGCGTTC255800.022.26988
TCAGACC286950.020.6762812
GCGTTCT289850.019.6847139
CTCAGAC327700.019.647351
GAACGAT303750.018.62595745
TTCGACG16800.017.5437766
TTCACGC293800.017.482657
TCACGCT304550.016.7030248
AATACGG338700.016.6204732
CCGGCTT252800.016.5718562
GCCGGTT73350.016.5367891
TACGGAG339200.016.4367134
CGTTCTC359650.016.32101410
ATACGGA345550.016.31049333
AGAACGA347750.016.30808644
Produced by FastQC (version 0.11.5)