FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55566050
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1118400.20127397934530167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT264000.020.63237845
AGACCGC318650.017.1598264
CAGACCG324100.016.9476433
AGAACGA322750.016.86970744
GACCGCG326350.016.7136295
CGTTACG26750.016.65334121
ACCGCGT328650.016.6490236
CCGCGTT345250.015.8745147
CGCGTTC346350.015.8305938
TTCGGCG81750.015.71472544
GTCCGTT47450.015.61245251
AACGCTA198550.014.7424823
TCAGACC378700.014.7002842
TCACGCT215750.014.6410828
ACGCTAC201000.014.57405524
CCGTACG87650.014.4022894
CCGGCTT237250.014.3785242
AAGAACG380400.014.34870243
GCGTTCT384900.014.3152119
CCCGTAT41600.014.23553851
Produced by FastQC (version 0.11.5)