FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48278646
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2650790.5490605515324518No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG675090.13983200771620644No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG571230.11831939114448238No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG529460.1096675329295689No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA525230.10879136916971531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT54550.028.4179976
GAACGAT513900.027.91011445
GATCGCG57850.026.9142075
AGACCGC512050.025.4943664
CAGACCG519400.025.1775443
GACCGCG521550.024.9523375
ACCGCGT529800.024.5674676
CGCGTTC595850.024.4317158
AGAACGA591600.024.2023144
CCGCGTT541550.024.0275617
GCGTTCT616850.023.6473759
TCAGACC570550.023.204312
TTCGACG27750.023.026316
TCGCGTT68800.022.5948567
CTCAGAC621000.022.2483351
AAGAACG655150.021.90526843
AATACGG671500.021.48681432
TACGGAG672300.021.45933734
CGTTCTC690700.021.42515210
ATACGGA677600.021.30540833
Produced by FastQC (version 0.11.5)