FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52644654
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG621720.11809746152002443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT326950.014.5064933
GGAGGTC353500.013.3405894
ATAGGGG237700.013.30860738
AGCTTAG246250.012.82810145
GTTGTCG32450.012.75789926
GGCATAG247800.012.62104435
GAGGTCA379550.012.5612685
ACTCTCC381000.011.78719111
TGGCATA269550.011.7278634
GTCACTC397100.011.5359218
GAGCTTA272700.011.50960944
GGTCACT414950.011.0451017
TCACTCT425250.010.8675229
CACTCTC422150.010.49429610
CATAGGG308450.010.24868137
TGGGAGG342250.010.0253512
TAGGGGA453650.09.92932339
GCATAGG325650.09.70749336
AGGGGAG468600.09.70377140
TCTCCCC468550.09.61826913
Produced by FastQC (version 0.11.5)