FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46573760
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG596130.12799696653222759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA157700.019.56961423
ACGCTAC159550.019.37106724
AGACCGC83150.019.2336464
CGCTACC166350.018.53868725
CAGACCG90050.018.1602253
AAAACGC171650.018.08297721
TTCGACG13850.018.0233126
TCACGCT180850.017.6824538
GAAAACG176550.017.62158620
TTCACGC184900.017.5140657
CCGGCTT200100.017.0767962
CGTTACG17150.016.93070621
CCGTACG51800.016.915524
GCCGGCT179650.016.812011
AAACGCT187900.016.66360522
CGTACGC52750.016.6535825
CCCGTAT29500.015.73936751
ACGCTCA215500.015.2671510
GAACGAT105850.014.99247745
TACGCCA61750.014.5674797
Produced by FastQC (version 0.11.5)