FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45556459
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1425810.31297647606895873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT301000.023.74028645
AGACCGC348850.021.103164
CAGACCG358650.020.60183
GACCGCG356950.020.5486645
ACCGCGT360650.020.3628966
CGCGTTC368150.019.9725068
CCGCGTT369750.019.8678677
AGAACGA364050.019.65341644
TTCGACG19950.018.8342746
TCAGACC401750.018.576442
GCGTTCT406900.018.1313029
CTCAGAC439650.017.9526731
AAGAACG412100.017.43830343
TACGGAG415450.017.0648934
AATACGG417950.017.03835132
ATACGGA419550.016.98929433
CAATACG432300.016.56124931
CGTTCTC464400.016.16736610
CGTACGC38350.016.0166135
ACGGAGA457450.015.55712735
Produced by FastQC (version 0.11.5)