FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55036747
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22100.017.2038966
CGTTACG16850.015.88918121
GTCCGTT45700.015.4161661
CCCGTAT44800.015.374171
GCCGGCT158350.014.9393921
AGACCGC98200.014.8241084
TTAGTTA71050.014.1855028
CAGACCG105950.013.9945413
CGCAAAC64000.013.99134725
CCGTACG33850.013.8919564
AACGCTA165150.013.62318523
ACGCTAC167850.013.36383224
TTCACGC178350.013.3583767
CCGGCTT205850.013.3565582
CTCCGTT67200.013.2974841
CCCCCCT46300.013.2718031
CCCGGAT122350.013.1353761
CTCCGAT50450.013.1169981
GTTCCTA78950.012.99397215
TCCGCAA69650.012.92123
Produced by FastQC (version 0.11.5)