FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56341911
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG787000.13968287302147064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG245750.015.48810545
GGGAGGT383900.014.0886393
ATAGGGG277700.013.71277138
GGCATAG276800.013.61753835
GAGCTTA286100.013.3509544
GTCACTC398150.012.9647628
GGTCACT398150.012.9195387
GGAGGTC429100.012.4842064
ACTCTCC424050.012.23640711
GTTGTCG26450.012.1617826
TGGCATA314300.012.13532234
CATAGGG330850.011.63870337
TCACTCT457900.011.4941659
CACTCTC454800.011.41897310
TGGGAGG366800.011.16820052
GCATAGG344950.011.110229536
AGGTCAC492300.010.85553556
GAGGTCA501100.010.7451155
GCTCTGT500700.010.43199122
TATCCTA101050.010.1981635
Produced by FastQC (version 0.11.5)