FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50105309
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT525710.10492101745146407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26450.022.0315116
GACCGCG127600.021.1075785
ACCGCGT127450.021.0782576
AGACCGC130900.020.7478854
CCGCGTT137500.019.5376287
CGCGTTC138250.019.4804618
CAGACCG143150.019.239613
CGGTATA40400.018.04568320
TCGACGA33450.017.421037
CCGGCTT194500.017.087052
GAACGAT159000.016.83914645
GCCGGCT172150.016.3907831
GTCCGTT33400.016.3707661
GTTCGAC34700.016.1459945
TTCACGC190100.016.0371577
CGACGAG35200.015.7239858
TCAGACC179500.015.5941632
TCACGCT194650.015.5235778
GCGTTCT180350.015.0827469
ACGCTCA207800.015.05010310
Produced by FastQC (version 0.11.5)