FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46537445
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1562800.3358155996746276No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG490220.1053388298390683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT305200.025.34364945
AGACCGC334650.023.583954
CAGACCG339650.023.2566433
GACCGCG349600.022.5754265
ACCGCGT353750.022.3042246
CCGCGTT362050.021.7929927
CGCGTTC365150.021.6079798
AGAACGA365150.021.47233644
TCAGACC393550.020.3230272
GCGTTCT389450.020.3117269
CTCAGAC426800.019.7060071
AATACGG411000.018.95179432
TACGGAG413800.018.76406934
ATACGGA417200.018.69196333
AAGAACG421000.018.59708843
CAATACG437200.018.01162731
ACGGAGA439450.017.71491835
CCCAATA446350.017.64792829
CGTTCTC455000.017.64756610
CGGAGAG449250.017.25335936
Produced by FastQC (version 0.11.5)