FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46537445
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG129700.012.86133745
ACCCGGC80300.012.4724295
GGCATAG138550.011.95723335
ATAGGGG144450.011.63701138
ATAGCTC81300.011.37575639
GGGAGGT235000.011.285143
GTCACTC218250.011.1364838
GAGCTTA156400.010.76703744
TCACCCG97250.010.7413253
TGGCATA159550.010.56691434
ACTCTCC233000.010.547873511
CATAGGG160300.010.36294537
CGTGGCG163500.010.290241
GGTCACT236050.010.2012237
TAGCTCT92250.010.19591740
TCAGACG90250.010.1291582
TAGCGTA7000.09.96836737
GCATAGG168300.09.95061936
CACCCGG107050.09.92587954
TCACTCT260300.09.8738049
Produced by FastQC (version 0.11.5)